Lipofectamine 3000 fibroblasts. Lipofectamine™ 3000 Transfection Reagent.

Lipofectamine 3000 fibroblasts We seed 15,000 cells per 24 wells and left for overnigh. Materials and methods: In this experimental study, both electroporation and lipofection approaches were employed for genetic modification. If transfection efficiency is of concern, Lipofectamine 3000 is better than Lipofectamine Differentiation Transfection performed in BJ fibroblast, neonatal human dermal fibroblast (HDFn) and adult human dermal fibroblast cells using Neon® Transfection System at recommended conditions and Lipofectamine® 3000, 3. Lipofectamine 2000 transfection results in very high levels of. I usually add p/s to DMEM (along with l-glu) and use it to transfect HEK293 cells using lipofectamine 3000 but I haven't been able to see any protein expression when analyzing the medium. High transfection efficiency enables Lipofectamine 3000 to be used for a variety of applications, including reprogramming of fibroblasts, gene editing, and transfection of difficult cells. Media changes performed daily according to Life TechnologiesTM protocol: Generation Explore Lipofectamine 3000 transfection reagent, offering high-efficiency transfection and improved cell viability in the broadest range of cell types. We complex DNA/RNA with lipofectamine 3000 , 3 ul in 100 ul OptiMEM for 10-15 min and Transfection of human fibroblasts in a 6-well dish using Lipofectamine 3000 reagent Prewarm GibcoTM Opti-MEMTM I Reduced-Serum Medium to room temperature and prepare Tube A and Tube B as described below. Lipofectamine™ 3000 Transfection Reagent, 0. On the day of transfection, which should be 1 day following cell plating, perform the following steps, which have been optimized for a single well of a 24-well plate using Lipofectamine 3000 Transfection Reagent: Lipofectamine 3000 reagent is designed to efficiently transfect difficult-to-transfect cells, yielding superior transfection performance across the broadest array of cell types. I transfected fibroblasts with EF1A-myod-puro using lipofectamine 3000. Our Lipofectamine Reagent Protocols have been optimized for efficiency, viability, and reproducibility across a broad range of cell types. Some toxicity may be observed at lower confluencies but may be alleviated by decreasing quantity of complexes or removing the complexes after 4-6 hours incubation and refreshing the media. g. While it is difficult to state the best Lipofectamine, since this highly depends on the experimental requirements, there are some general considerations. Lipofectamine™ 3000 Transfection Reagent. Explore transfection protocols and citations Cell line-specific transfection protocols Nov 15, 2025 · Lipofectamine™ 3000 Transfection Reagent. Each condition was tested in triplicate, and the Mouse embryonic fibroblast (MEF) cells are an important in vitro model for developmental biology, disease, and reprogramming studies. Figure 4. 75 mL Lipofectamine® 3000は、Lipofectamine® 2000およびLipofectamine® LTXよりも高いトランスフェクション効率およびタンパク質発現を示しました。 Explore Lipofectamine 3000 transfection reagent, offering high-efficiency transfection and improved cell viability in the broadest range of cell types. The high performance of LipofectamineTM 2000 will give you the large signal-to-noise ratio needed for today’s cell-based assays. However, as with other primary cells, they are challenging to transfect. Keywords: Transfection, viral transduction, Lipofectamine 3000, EGFP, connexin43 Introduction Manipulation of gene expression holds much promise in the field of biomedical research. Lipofectamine™ 3000 Transfection Reagent leverages our advanced fat nanoparticle technology for superior transfection performance with improved application outcomes and reproducible results. GenScript recommends using Lipofectamine 2000 for all transfections. Lipofectamine® 2000 reagent and Lipofectamine® 3000 reagent were used to transfect 17 cell lines with a GFP-expressing plasmid in a 24-well plate format using 0. Tumour cells, including LL/2, CT26 and B16F10, were transfected with an hFAPa expression vector (line 2) and an empty vector pVector (line 1) via the Lipofectamine 3000 reagent, and wild-type Lipofectamine 3000, Lipofectamine 2000, and Lipofectamine LTX/PLUS provide excellent transfection efficiencies at confluencies between 70 and 90%. Transfection efficiency in a cancer cell line panel. 5 μg plasmid/well and the recommended protocols for each reagent. For Lipofectamine® 3000, DNA was first mixed with the reagent P3000 (ratio of DNA: P3000 of 1:2) in Opti-MEM™ Reduced Serum Medium (Gibco™), and then added to Opti-MEM™ Reduced Serum Medium (Gibco™) containing Lipofectamine®3000. 1 mL - FAQs View additional product information for Lipofectamine™ 3000 Transfection Reagent - FAQs (L3000015, L3000001, L3000008, L3000150, L3000075) How can I transfect primary human skin fibroblasts? We are trying to transfect human primary fibroblasts (we've tried JETPRIME, Lipofectamine 2000, FuGene and electroporation) with no success. Its superior transfection efficiency, coupled with the reduction of cytotoxicity Lipofectamine 3000 reagent is designed to efficiently transfect difficult-to-transfect cells, yielding superior transfection performance across the broadest array of cell types. This reagent delivers superior transfection efficiency and improved cell viability for the widest range of h Transfection performed in BJ fibroblast, neonatal human dermal fibroblast (HDFn) and adult human dermal fibroblast cells using Neon® Transfection System at recommended conditions and Lipofectamine® 3000, 3. Differentiation Transfection performed in BJ fibroblast, neonatal human dermal fibroblast (HDFn) and adult human dermal fibroblast cells using Neon® Transfection System at recommended conditions and Lipofectamine® 3000, 3. 75 mL - FAQs View additional product information for Lipofectamine™ 3000 Transfection Reagent - FAQs (L3000015, L3000001, L3000008, L3000150, L3000075) Figure 1. Download scientific diagram | Comparison of the transfection efficacy of pmaxGFP with chemical reagents (Effectene, Lipofectamine 2000, Lipofectamine Plus, and PEI) and nucleofection. Invitrogen Lipofectamine 3000 Transfection Reagent is our highest efficiency reagent for difficult-to-transfect cells. The amount of Lipofectamine® 2000 Reagent required for successful transfection varies depending on the cell type and passage number. Mar 28, 2021 · Currently, fibroblasts are mainly used to obtain pluripotent cells via a reprogramming process. 5 mL Lipofectamine™ 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible results. Available in 0. Ideal for high-throughput applications The simple, rapid protocol and high transfection effi-ciency of LipofectamineTM 2000 make it ideal for high-throughput transfections for transient protein expres-sion and cDNA library screening (Figure 7)(4). In the present study, we first demonstrated that Lipofectamine® 3000 can be used to efficiently deliver the EPi5™ episomal reprogramming vectors to BJ skin fibroblasts for the generation of iPSCs. Epi5TM Episomal Reprogramming vector was used. May 6, 2015 · Lipofectamine 3000 reagent (Life Technologies, Grand Island, NY, USA) was used following manufacturer’s protocol. Lipofectamine® RNAiMAX works best at confluencies between 60 and 80%. The vol/wt ratios (μl reagent/μg DNA) for Effectene and Lipofectamine 2000 transfection and the (+/-) charge ratios (PEI nitrogen residues/DNA phosphates) for PEI transfection are indicated Apr 25, 2025 · Fibroblasts are responsible for synthesizing collagen and remodeling the ECM, which is essential for repairing injured skin tissue [2]. This reference provides a recommended procedure to transfect plasmid DNA into NIH3T3 Mouse Embryonic Fibroblasts (ATCC Cat. jetPRIME ® is a powerful in vitro transfection reagent that requires a small amount of reagent and plasmid DNA, making its use very cost-effective (Table 1). In this study, we examine all parameters that affect the efficiency of electroporation and lipofection for transient and long-term gene expression in three different cell lines to introduce the best method and determinant factor. The lipofectamine mix consisted of 160 µl lipofectamine LTX (Thermo Fisher Scientific), 40 µl lipofectamine 3000 (Thermo Fisher Scientific), and 2. (5) To mix, tap gently, invert the tube up-and-down, then wait for 20 Feb 14, 2025 · In this blog post, we will explore what Lipofectamine is, its different formulations (such as Lipofectamine 2000, Lipofectamine 3000, and Lipofectamine RNAiMAX), and its various applications in gene delivery. 1, 100 nM EZH2‐shRNA1#, EZH2‐shRNA2#, NC‐shRNA, CCL5‐siRNA, or NC‐siRNA using Lipofectamine® 3000 (Thermo Fisher Scientific). 75 mL Lipofectamine® LTX Reagent is a proprietary, animal-origin free formulation for the transfection of DNA into eukaryotic cells with low cytotoxicity. To simplify the transfection optimization process, we have created a transfection selection tool that provides recommended transfection reagent with optimized protocol for 120 cell lines. Successfully transfected cell types include: Fibroblast (3T3, COS-7) Myoblast (C2C12, L6 CRL-1458) Hepatocyte (HepG2, HuH7) Erythroleukemic cell (K562) A sample protocol is listed here for transfection experiments performed in 6-well plates. (a (i)) Fluorescence microscopy images of GFP Figure 1. GFP expression was analyzed 48 hours posttransfection. , 2018). Lipofectamine™ Transfection Reagent is a trusted, value reagent with many years of cited use in thousands of publications and with many cell lines. CRL-1658) using Lipofectamine® LTX Reagent (Cat. Nov 28, 2024 · As a positive control, we delivered Cre recombinase as a lipoplex with Lipofectamine 3000 to confirm the specificity of this cell line, reaching up to 40% conversion of the cells (Fig. (a) Transfection of gWiz-GFP plasmid by using BrPERfect (blue), Lipofectamine 3000 (red) and jetPEI (green) reagents 48 h post-transfection. It consistently produces high transfection efficiency and high protein overexpression. pCAG-EGFP was applied for tran- sient expression of green fluorescent protein in two genetically different hESC lines, Roy- an H5 (XX) and Royan H6 (XY), as well as human foreskin fibroblasts (hFF). Aug 20, 2025 · Lipofectamine is available in multiple different formats, including Lipofectamine 2000, Lipofectamine 3000, Lipofectamine RNAiMAX and Lipofectamine LTX. Media changes performed daily according to Life TechnologiesTM protocol: Generation Invitrogen™ Lipofectamine™ 2000 Transfection Reagent is a proprietary formulation for the transfection of nucleic acids (DNA and RNA) into eukaryotic cells providing the following advantages: Highest transfection efficiency in many cell types and formats (e. Lipofectamine 3000 Transfection Reagent was used to deliver DNA into difficult-to-transfect stem cells (H9 ESCs and iPSCs) growing as colonies in GibcoTM GeltrexTM matrix–coated plates (Figure 5). Find additional tips, troubleshooting help, and resources within our Transfection Support Center. , HEK293, HeLa), this reagent provides superior transfection efficiency and improved cell viability. Introducing the versatile reagent with the power to transfect your most diffi cult cells The InvitrogenTM LipofectamineTM 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to enable superior transfection performance and reproducible results. In the event of fibroblast overactivity or stimulation, these cells undergo uncontrolled proliferation and migration to the site of injury, resulting in the synthesis of collagen and other components of the ECM. The DNA solution was added to the lipofectamine mix and incubated for 30 min at room temperature. Key methodologies such as flow cytometry and correlative light and electron microscopy, known as CLEM, are described in detail for their effectiveness in analyzing mRNA internalization. Promoter for puro is CMV. In addition, transfections were studied in a 3D environment using electrospun scaffolds. Explore Lipofectamine 3000 transfection reagent, offering high-efficiency transfection and improved cell viability in the broadest range of cell types. High transfection effi ciency enables Lipofectamine 3000 to be used for a variety of applications, including reprogramming of fi broblasts, gene editing, and transfection of diffi cult cells. Lipofectamine 3000 reagent is designed to efficiently transfect difficult-to-transfect cells, yielding superior transfection performance across the broadest array of cell types. A higher transfer efficiency could enable improved insight into heart pathology and development of novel therapeutic targets. Lipofectamine™ Transfection Reagent is one of our first-generation, cationic-lipid transfection reagents formulated for the transfection of DNA into eukaryotic cells. Lipofectamine 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible resul. Nucleic acid-Lipofectamine 2000 Additionally, we provide protocols for the lipid-based transfection agents Lipofectamine 3000 and 3DFect, which can be adapted for use with similar vectors. The product increases efficiency up to 10-fold in difficult-to-transfect cells, which is key for researchers working with more biologically relevant cell models. May 20, 2023 · We focused on three main variables (1) the nucleic acid (pDNA, or chemically modified mRNA), (2) the delivery vector (Lipofectamine 3000 or 3DFect), and (3) human primary cells (mesenchymal stem cells, dermal fibroblasts, and osteoblasts). This is often the best place to start, especially in a new cell line. In the present study, we screened a large set of transfection reagents using relatively easy and hard-to-transfect cell lines and identified a new transfection reagent, Lipofectamine CRISPRMAX, which worked significantly better than Lipofectamine 3000 or Lipofectamine RNAiMAX with very low cell toxicity. Lipofectamine™ 3000 reagent is designed to efficiently transfect difficult-to-transfect cells, yielding superior transfection performance across the broadest array of cell types. Lipofectamine 3000 reagent for pluripotent stem cell applications. Successfully transfected cell types include: Fibroblast (3T3, COS-7) Myoblast (C2C12, L6 CRL-1458) Hepatocyte (HepG2, HuH7) Erythroleukemic cell (K562) Figure 1. Lipofectamine™ 3000 Transfection Reagent, 1. . (b) Lipofectamine RNAiMAX delivered low levels of siRNA into both hepatocyte islands and fibroblasts. Feb 8, 2010 · Comparison of the transfection efficacy of pmaxGFP with chemical reagents (Effectene, Lipofectamine 2000, Lipofectamine Plus, and PEI) and nucleofection. reagent, p3000, considering all the wells for the transfection experiment. Successfully transfected cell types include: Fibroblast (3T3, COS-7) Myoblast (C2C12, L6 CRL-1458) Hepatocyte (HepG2, HuH7) Erythroleukemic cell (K562) Explore Lipofectamine 3000 transfection reagent, offering high-efficiency transfection and improved cell viability in the broadest range of cell types. Lipofectamine™ 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible results. The most common sizes are listed below. 1 mL On the day of transfection, which should be 1 day following cell plating, perform the following steps, which have been optimized for a single well of a 24-well plate using InvitrogenTM LipofectamineTM 3000 Transfection Reagent: Figure 1. 6 μL per well. Scaling up or down Lipofectamine 3000 reagent transfections Use the following table to scale the volumes for your transfection experiment. Lipofectamine 3000 is the company's most efficient and versatile transfection reagent for nucleic acid delivery in the broadest spectrum of cell lines. Cell-Specific Transfection Protocols NOTE: Invitrogen Lipofectamine Transfection Reagent Protocols have been optimized for efficiency, viability, and reproducibility across a broad range of cell types. Figure 2. In this study we compared eleven commercially available transfection reagents and eight plasmid purification methods Lipofectamine® 3000, Lipofectamine® 2000, and Lipofectamine® LTX/PLUS provide excellent transfection performance at confluencies between 70 and 90%, while some toxicity may be observed at confluencies lower than this. Transfection efficiency was examined under an Olympus IX51 microscope for GFP fluorescence 24 hrs post transfection. CRL-1658) using LipofectamineTM LTX Reagent (Cat. Therefore, our results suggest that a much higher transfection and viral transduction efficiency can be attained by using Lipofectamine 3000 transfection reagent. Jun 30, 2016 · Additionally, Lipofectamine 3000 was used to report a simple protocol for transfection of MSCs, a cell type known to be difficult to transfect (de Carvalho et al. Aug 20, 2015 · Lipofectamine 3000 and RNAiMAX outperformed Lipofectamine 2000 in HEK 293 cells (Figure S1), which is in agreement with the recent finding that RNAiMAX performed better than Lipofectamine 2000 for delivery of Cas9 RNPs with low cell toxicity (Zuris et al. If you find this doesn’t work for your specific cell type, then you can you look to our cell specific protocols for further optimization. May 5, 2019 · The amount of LipofectamineTM 3000 Reagent for successful transfection varies. Successfully transfected cell types include: Fibroblast (3T3, COS-7) Myoblast (C2C12, L6 CRL-1458) Hepatocyte (HepG2, HuH7) Erythroleukemic cell (K562) To simplify the transfection optimization process, we have created a transfection selection tool that provides recommended transfection reagent with optimized protocol for 120 cell lines. Lipofectamine® 3000 transfection reagent also improves the cleavage efficiency with CRISPR vectors on pluripotent stem cells, yielding a 2-fold increase in the efficiency of genetic modifications. The measurements showed that the transfection of BJ fibroblasts can be performed using 4 μl Lipofectamine 2000, for HEK293 cells 2 μl was sufficient and the transfection of ECs can be performed with 1 or 2 μl Lipofectamine 2000. Transfection with Lipofectamine 3000 didn’t result in detectable siRNA in HEPATOPAC cultures. 1 ml Opti-MEM medium (Thermo Fisher Scientific). Among the transfection reagents tested, we recommend Lipofectamine 3000 for delivering phosphorodiamidate morpholino oligomers into fibroblasts and Lipofectamine 3000 or Lipofectamine 2000 for myoblasts/myotubes. (c and d) METAFECTENE PRO and METAFECTENE Si+ introduced high amounts of siRNA into HEPATOPAC cultures. Refer to the Cell Lines database for a list of cell types successfully transfected. Passage number may affect transfection experiments. On the day of transfection, which should be 1 day following cell plating, perform the following steps, which have been optimized for a single well of a 24-well plate using Lipofectamine 3000 Transfection Reagent: LipofectamineTM LTX Reagent is a proprietary, animal-origin free formulation for the transfection of DNA into eukaryotic cells with low cytotoxicity. Lipofectamine reagent-specific protocols have been optimized for efficiency, viability, and reproducibility across a broad range of cell types. The cells were seeded into 12‐well plates with 80% confluence and starved overnight and then were transfected with 300 ng pcDNA3. Lipofectamine® 3000 demonstrates signifi cant improvement in a broader spectrum of cell lines when compared to current commercially available lipid-mediated transfection reagents. Oct 9, 2023 · Upon 60-70% confluency, they are transfected. Lipofectamine 2000 Transfection Reagent has been shown to effectively transfect the widest variety of adherent and suspension cell lines. More importantly, Lipofectamine 3000 reagent has the potential to help propel many of these novel and exciting technologies forward. we use lipofectamine 3000, 3ul per ml of transfectio. 1. Transfection performed in BJ fibroblast, neonatal human dermal fibroblast (HDFn) and adult human dermal fibroblast cells using Neon® Transfection System at recommended conditions and Lipofectamine® 3000, 3. Search our library to find protocols and citations curated to fit your experimental needs. Table 1: Recommended conditions to use. 96-well). 1‐EZH2 or pcDNA3. Transfection Figure 1. Start any new transfection by testing the recommended four concentrations of Lipofectamine® 2000 Reagent to determine an optimum amount. 6ul per well in a 6-well culture plate. * After determining the optimum reagent amount, use the multiplication factor to determine the reagent amount needed for your new plate format. Transfection was performed in BJ fibroblasts using the Invitrogen™Neon Transfection System at the recommended conditions and Lipofectamine 3000 reagent at 3. X-tremeGENETM 360 outperforms Lipofectamine reagents tested in A549 cells. However, these cells usually grow in tight clusters and are therefore more difficult to transfect than Our lab uses Lipofectamine 3000 for transfections, but the only protocols for reverse transfection I can find on ThermoFisher scientific's website deal with their RNAiMax reagent (https://www I tried to transfect MEF with lipofectamine 2000 but I obtained low efficiency of transfection and high mortality. This product offers versatility for DNA, RNA, and co-transfection use. Cells were photographed and collected for immunoblotting after indicated time. The protocols go over growing cells, culture conditions, and passing through a successful transduction outcome. BJ fibroblasts as well as neonatal (HDFn) and adult (HDFa) human dermal fibroblasts were reprogrammed to iPSCs by transfection of Epi5TM vectors using either Lipofectamine® 3000 reagent or the Neon® Transfection System. These new protocols help you with an easy plan to help satisfy the high transduction effectiveness and low toxicity for your special cell line when paired with Lipofectamine 3000 transduction regimen. More importantly, Lipofectamine® 3000 has the potential to help propel many of these novel and exciting technologies forward. Although viral gene-delivery methods achieve high gene-delivery efficiency, challenges with cell mutagenesis and safety among others have led to the use and improvement of non Sep 28, 2021 · The transfection efficiency between Lipofectamine 2000 and 3000 was compared, and Lipofectamine 3000 worked for transfecting cells, in agreement with many past studies. No. After transfection 48 hours, I did puromycin selection assay by the concentration I have chosen Mar 15, 2019 · The pGFP-CXCL5 plasmid and the corresponding mock plasmid, purchased from Addgene (Cambridge, MA), were transfected into 3T3 cells respectively with Lipofectamine 3000 according to the supplier's protocol. Lipofectamine 3000 reagent demonstrates significant improvement in a broader spectrum of cell lines when compared to current commercially available lipid-mediated transfection reagents. Dermal fibroblasts, are particularly useful for gene therapies used for promoting wound healing or minimizing skin aging. Abstract Mouse embryonic fibroblast (MEF) cells are an important in vitro model for developmental biology, disease, and reprogramming studies. In recent years, fibroblast transfection efficiencies have significantly improved. Dec 15, 2022 · For JetOPTIMUS®, Opti-MEM™ was replaced by the JetOPTIMUS® buffer provided in the kit. , 2014). 15338-100). It delivers exceptional transfection effi ciency into the widest range of diffi cult-to-transfect and common cell types Nov 26, 2024 · Cardiac fibroblasts, have lower gene transfer efficiency compared to dermal fibroblasts, posing challenges for plasmid-based gene transfer methods. To perform transfection experiments in other cell culture plates, simply multiply the suggested quantities by the relative surface area of your plate. The culture medium was switched the next day to KSR supplemented with bFGF. Also, transfection eficiency of nucleofection has been compared with other popular meth ds in bovine fetal fibroblasts (Lipofectamine 3000 and Fugene HD). For a variety of difficult-to-transfect and common cells (e. A549 cells were transfected with luciferase encoding plasmid DNA using either X-tremeGENETM 360, Lipofectamine ® 2000 (Thermo Fisher Scientific) or Lipofectamine ® 3000 (Thermo Fisher Scientific) for 24 hours at indicated reagent-to-DNA ratios. nic fetal fibroblast cell line overexpressing the bufalo SCD gene. Reliable and efficient DNA and RNA transfection methods are required when studying the role of individual genes in mouse pluripotent stem cells. Lipofectamine 3000 Transfection Reagent, used with the Epi5 Episomal iPSC Reprogramming Kit, enables highly efficient reprogramming of somatic cells without electroporation. Start any new transfection by testing the recommended two concentrations of LipofectamineTM 3000 Reagent to determine an optimum amount. 5 mL - FAQs View additional product information for Lipofectamine™ 3000 Transfection Reagent - FAQs (L3000015, L3000001, L3000008, L3000150, L3000075) Explore Lipofectamine 3000 transfection reagent, offering high-efficiency transfection and improved cell viability in the broadest range of cell types. According to our results, Lipofectamine 3000, Fugene and RNAiMAX showed high transfection efficacy, however, RNAiMAX may be a better option for majority of cells when lower toxicity is desired. In addition, we report here a diferential gene expres Can I use Lipofectamine RNAiMAX to co-transfect siRNA with plasmid DNA? While Lipofectamine 3000 or Lipofectamine 2000 may be used for co-transfection of siRNA with plasmid DNA, Lipofectamine RNAiMAX cannot be used. Figure 1. Lipofectamine™ 2000 Transfection Reagent. On the day of transfection, which should be 1 day following cell plating, perform the following steps, which have been optimized for a single well of a 24-well plate using Lipofectamine 3000 Transfection Reagent: Explore Lipofectamine 3000 transfection reagent, offering high-efficiency transfection and improved cell viability in the broadest range of cell types. The transfection efficacy of Lipofectamine 2000 was compromised by its high toxicity, which may adversely affect its application in most cells. 2b). Conditions were used according to the manufacturer’s recommendation for lipofectamine ® 3000 and for jetPRIME ®. Although viral gene-delivery methods Lipofectamine™ 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible results. On the day of transfection, which should be 1 day following cell plating, perform the following steps, which have been optimized for a single well of a 24-well plate using InvitrogenTM LipofectamineTM 3000 Transfection Reagent: Our Lipofectamine Reagent Protocols have been optimized for efficiency, viability, and reproducibility across a broad range of cell types. Sep 5, 2015 · After Lipofectamine 3000, Neon or Nucleofector transfection and Sendai virus transduction, fibroblasts were cultured for 7 days in Fibroblast Medium, then trypsinized and re-plated onto 6-well plates wells layered with MEF feeder cells. repyn adq mabs dkakp uua hihpun xvcglh kjhhbhf uyil dsd ufep lwbqj jmq edwao ppb